Author/Authors :
Watanabe، نويسنده , , Takafumi and Shinohara، نويسنده , , Nobuo and Sazawa، نويسنده , , Ataru and Kobayashi، نويسنده , , Yoshiyasu and Ogiso، نويسنده , , Yoshifumi and Kimura، نويسنده , , Takashi and Takiguchi، نويسنده , , Mitsuyoshi and Yasuda، نويسنده , , Jun and Hashimoto، نويسنده , , Akira and Koyanagi، نويسنده , , Tomohiko and Kuzumaki، نويسنده , , Noboru، نويسنده ,
Abstract :
To investigate the suppressive effect of dominant negative H-ras mutant N116Y on transformed phenotypes, we established two N116Y ras mutant stable transfectant clones (C5, C13) of human bladder cancer cell line, UMUC-2. These N116Y ras mutant transfectants, especially the C5 cells, showed a dramatic change of cellular morphology and significantly reduced growth in soft agar compared to their control. Furthermore, phosphorylation of the Jun NH2-terminal kinase (JNK) was significantly decreased in these transfectants compared to the control. These results suggest that the N116Y-induced suppression of transformed phenotypes in UMUC-2 cells is associated with inhibition of JNK phosphorylation.
Keywords :
bladder cancer , Transformed phenotype , Jun NH2-terminal kinase , Gene Therapy , Dominant negative H-ras mutant
