Author/Authors :
Pekarsky، نويسنده , , Yuri and Zabarovsky، نويسنده , , Eugene and Kashuba، نويسنده , , Vladimir and Drabkin، نويسنده , , Harry and Sandberg، نويسنده , , Avery A. and Morgan، نويسنده , , Rodman and Rynditch، نويسنده , , Alla and Gardiner، نويسنده , , Katheleen، نويسنده ,
Abstract :
Rearrangements of band 3q21 have been well documented in leukemia. To analyze the region involved, we have isolated a normal genomic P1 clone that spans the 3q21 breakpoints derived from two leukemia patients carrying t(3;3)(q21;q26). Both breakpoints are contained within a 50-kb NotI restriction fragment, but are not identical. They are separated by 9–11 kb and may disrupt genes associated with either the proximal or distal NotI sites. We further show that one patient carried a small insertion of material from chromosome 2 in the 3q - derivative. The P1 clone will allow isolation of candidate genes for these breakpoints and investigations into the clustering of other 3q21 leukemia breakpoints.
