Author/Authors :
Frye، نويسنده , , Michaela and Dragoni، نويسنده , , Ilaria and Chin، نويسنده , , Suet-Feung and Spiteri، نويسنده , , Immaculada and Kurowski، نويسنده , , Agata and Provenzano، نويسنده , , Elena and Green، نويسنده , , Andrew and Ellis، نويسنده , , Ian O. and Grimmer، نويسنده , , Donna and Teschendorff، نويسنده , , Andrew and Zouboulis، نويسنده , , Christos C. and Caldas، نويسنده , , Carlos and Watt، نويسنده , , Fiona M.، نويسنده ,
Abstract :
We have examined expression of the Myc target gene Misu (NSUN2) in breast cancer. There was extensive copy number gain, and increased mRNA and protein levels, of Misu in approximately one third of breast cancer cell lines and primary tumours examined, irrespective of tumour subtype. Genes on 5p15.31-33, where Misu is located, showed evolutionary synteny. siRNA-mediated knockdown of Misu reduced cell number in over half of the cell lines tested, irrespective of estrogen receptor status. We conclude that Misu is up-regulated in a substantial proportion of breast cancers and has therapeutic potential as a drug target.
Keywords :
RNA methyltransferase , MYC , gene amplification , breast cancer
