Author/Authors :
Daskalos، نويسنده , , Alexandros and Logotheti، نويسنده , , Stella and Markopoulou، نويسنده , , Soultana and Xinarianos، نويسنده , , George and Gosney، نويسنده , , John R. and Kastania، نويسنده , , Anastasia N. and Zoumpourlis، نويسنده , , Vassilis and Field، نويسنده , , John K. and Liloglou، نويسنده , , Triantafillos Liloglou، نويسنده ,
Abstract :
p73 possesses an extrinsic P1 promoter and an intrinsic P2 promoter controlling the expression of the pro-apoptotic TAp73 isoforms and the anti-apoptotic ΔΝp73 isoforms respectively. In this study, we investigated the DNA methylation status of both promoters as a means of epigenetic transcriptional control of their corresponding isoforms in 102 primary non-small cell lung carcinomas (NSCLCs). We demonstrated that while P1 hypermethylation-associated reduction of TAp73 mRNA levels is relatively infrequent, the P2 hypomethylation-associated over-expression of ΔΝp73 mRNA is a frequent event, particularly among squamous cell carcinomas. P2 hypomethylation strongly correlated with LINE-1 element hypomethylation, indicating that ΔΝp73 over-expression may be a passive consequence of global DNA hypomethylation.
Keywords :
p73 , lung cancer , TAp73 , DNA methylation , ??p73
