Author/Authors :
Ma، نويسنده , , Jie and Wang، نويسنده , , Shengjun and Ma، نويسنده , , Chi-Bin and Mao، نويسنده , , Chaoming and Tong، نويسنده , , Jia and Yang، نويسنده , , Min and Wu، نويسنده , , Chaoyang and Jiao، نويسنده , , Zhijun and Lu، نويسنده , , Liwei and Xu، نويسنده , , Huaxi، نويسنده ,
Abstract :
Glucocorticoid-induced tumor necrosis factor receptor and its ligand (GITRL) are critically involved in the regulation of immune response. In this study, we aimed to generate bone marrow-derived dendritic cells (BMDCs) transfected with recombinant adenovirus expressing GITRL (pAd-GITRL-BMDCs) and explore their therapeutic efficacy in murine Lewis lung carcinoma. In vitro, pAd-GITRL-BMDCs greatly enhanced effector T cells proliferation but markedly abrogate the suppression of Treg cells. Moreover, vaccination with pAd-GITRL-BMDCs significantly retarded tumor growth, which was accompanied with increased IFN-γ-producing CD8+ T cells and markedly decreased Treg cells in vivo. These findings suggest GITRL could enhance the immune stimulation of DC and might facilitate the potential development of DCs-based anti-tumor therapies.
