Incorporation of cytochrome C with thin calcium phosphate film formed by electron-beam evaporation

Surface properties play a major role to determine the biocompatibility that is the ability of a material to perform with an appropriate host response in a specific application because biomaterials contact and interact with biological systems. Various methods have been studied to improve the surface properties and farther the osseous intergradation of bone implants. Cytochrome C (cyt C) was immobilized on the thin calcium phosphate films formed by electron-beam evaporation in the form of cyt C-apatite composite layer. The newly formed apatite layer was observed by scanning electron microscopy, and X-ray photoelectron spectroscopy confirmed that cyt C existed in the newly formed layer. Cyt C released from the cyt C-apatite composite layer for at least 10 days in the physiological salt solution.