Author/Authors :
Carlo M. and Fernلndez-Piqueras، نويسنده , , José and Santos، نويسنده , , Javier and Pérez de Castro، نويسنده , , Ignacio and Meléndez، نويسنده , , Bلrbara and Martيnez، نويسنده , , Beatrيz and Robledo، نويسنده , , Mercedes and Rivas، نويسنده , , Carmen and Benيtez، نويسنده , , Javier، نويسنده ,
Abstract :
We present an allelotype analysis of 35 cases of non-Hodgkin lymphomas and normal pairs using four microsatellite markers that flank the region occupied by the CDKN2 gene locus at 9p21. Frequent allelic losses (LOH) were detected in B-cell lineage NHLs, including Burkitt lymphoma (33.3% of total, if we only consider high grade tumors). In five of these tumors LOH did not include the CDKN2 gene. Mutational analysis of exon 1 and 2 of CDKN2 (SSCP and sequencing of abnormal bands) revealed a nonsense mutation (Arg72Ter) in one tumor (case 10), where the second hit of the Knudsonʹs model consisted of the elimination of the wild type allele. In view of these results, the hypothesis of two different candidate tumor suppressor gene regions around the CDKN2 locus remains an intriguing possibility.
