Author/Authors :
Sueyoshi، نويسنده , , Takanao and Jono، نويسنده , , Hirofumi and Shinriki، نويسنده , , Satoru and Ota، نويسنده , , Kazutoshi and Ota، نويسنده , , Tomoko and Tasaki، نويسنده , , Masayoshi and Atsuyama، نويسنده , , Eri and Yakushiji، نويسنده , , Toshitake and Ueda، نويسنده , , Mitsuharu and Obayashi، نويسنده , , Konen and Mizuta، نويسنده , , Hiroshi and Ando، نويسنده , , Yukio، نويسنده ,
Abstract :
Midkine (MK) plays important roles in tumorigenesis, however, the biological function of MK and whether MK can be a therapeutic target in osteosarcoma are unclear. Here, we found that osteosarcoma tissues showed high MK expression. MK knockdown by small interfering RNA significantly induced apoptosis in osteosarcoma cells, whereas recombinant MK increased cell proliferation. Inhibition of MK signaling by anti-MK monoclonal antibody (anti-MK mAb) suppressed growth of osteosarcoma cells both in vitro and in vivo. Moreover, inhibition of MK function significantly suppressed lung metastasis in xenograft transplantation model. Targeting MK by anti-MK mAb may have value in the treatment of osteosarcoma.
Keywords :
Anti-midkine antibody , Midkine , Osteosarcoma , Cell Proliferation , Lung metastasis
