Title of article :
Survivin isoform Delta Ex3 regulates tumor spheroid formation
Author/Authors :
Espinosa، نويسنده , , Magali and Ceballos-Cancino، نويسنده , , Gisela and Callaghan، نويسنده , , Richard and Maldonado، نويسنده , , Vilma and Patiٌo، نويسنده , , Nelly and Ruيz، نويسنده , , Vيctor and Meléndez-Zajgla، نويسنده , , Jorge، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Pages :
7
From page :
61
To page :
67
Abstract :
Survivin is an important member of the Inhibitor of Apoptosis Proteins (IAPs) family and has essential roles in apoptosis and cell cycle progression. This gene is commonly upregulated in human cancer and provides an exciting diagnostic and therapeutic target. Survivin is expressed as several isoforms that are generated by alternative splicing, and some of these present antagonistic activities. Currently, information regarding the regulation of these isoforms is lacking. In this study, we sought to analyze survivin Delta Ex3 expression in a three-dimensional model of avascular tumors and its overexpression effects in processes such as proliferation, clonogenicity and apoptosis. We found a positive correlation between spheroid growth and survivin Delta Ex3 expression during the exponential phase. We demonstrated that this isoform not only decreased apoptosis but also inhibited tumor spheroid formation by decreasing proliferation and clonogenic survival. These results point toward a dual and antagonistic effect of this spliced survivin isoform in cancer development.
Keywords :
Spheroid , apoptosis , Survivin , cancer
Journal title :
Cancer Letters
Serial Year :
2012
Journal title :
Cancer Letters
Record number :
1821187
Link To Document :
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