Author/Authors :
Muresu، نويسنده , , Rosella and Cossu، نويسنده , , Antonio and Scarpa، نويسنده , , Anna Maria and Volpi، نويسنده , , Emanuela Valeria and Rocca، نويسنده , , Paolo Cossu and Pintus، نويسنده , , Adriana and Tibiletti، نويسنده , , Maria Grazia and Tanda، نويسنده , , Francesco and Pirastu، نويسنده , , Mario and Massarelli، نويسنده , , Giovannino، نويسنده ,
Abstract :
Retrospective analysis of chromosomal changes in endometrial carcinoma was performed by fluorescence in situ hybridization on free nuclei isolated from formalin-fixed paraffin-embedded tissue. We examined 23 archival samples for numerical aberrations of chromosomes 1 and 10 with the use of specific DNA probes for the pericentromeric and centromeric regions of these two chromosomes. Numerical aberrations of chromosomes 1 and 10 were detected in 39% of the case analyzed, and the frequency of trisomy 10 tended to increase as the histological grade worsened. Our findings confirm the association of cytogenetic anomalies involving chromosomes 1 and 10 with endometrial carcinoma, as reported by other studies, and suggest that changes in centromere 10 copy number may correlate with the degree of tumor differentiation.
