Author/Authors :
Ruan، نويسنده , , Qing and Xi، نويسنده , , Lei and Boye، نويسنده , , Sanford L. and Han، نويسنده , , Song and Chen، نويسنده , , Zhi J. and Hauswirth، نويسنده , , William W. and Lewin، نويسنده , , Alfed S. and Boulton، نويسنده , , Michael E. and Law، نويسنده , , Brian K. and Jiang، نويسنده , , Wen G. and Jiang، نويسنده , , Huabei and Cai، نويسنده , , Jun، نويسنده ,
Abstract :
We aimed to develop an anti-angiogenic model for breast cancer by combining (1) siRNA-based therapy delivered by self-complementary adeno-associated virus serotype 2 (scAAV2) vectors to target tumor vasculature, and (2) noninvasive monitoring to tumor response to anti-angiogenesis by photoacoustic (PA) imaging. scAAV2 vector containing 7 surface exposed tyrosine to phenylanine capsid mutations was able to transduce microvascular endothelial cells with high efficiency. siRNAs against UPR (unfolded protein response)-IRE1α, XBP-1, ATF6 significantly inhibited breast cancer-induced angiogenesis in vitro by inhibiting endothelial cell survival. PA imaging showed that knockdown of UPR proteins greatly reduced tumor angiogenesis in vivo in breast cancer models.
Keywords :
Photoacoustic imaging , unfolded protein response , Angiogenesis , breast cancer , scAAV2 , siRNA
