Author/Authors :
Sirchia، نويسنده , , Silvia M and Sironi، نويسنده , , Elena and Grati، نويسنده , , Francesca R and Serafini، نويسنده , , Paola and Garagiola، نويسنده , , Isabella and Rossella، نويسنده , , Franca and Dulcetti، نويسنده , , Francesca and Pardi، نويسنده , , Giorgio and Garsia، نويسنده , , Salvatore and Simoni، نويسنده , , Giuseppe، نويسنده ,
Abstract :
We analyzed 37 samples of endometrial adenocarcinoma for loss of heterozygosity (LOH) by using a panel of 44 microsatellites located in 29 chromosomal regions. The aim of our study was to investigate the existence of a possible preferential involvement of some tumor suppressor genes in endometrial carcinogenesis. The analysis was performed on tumoral tissue and on a corresponding normal tissue by the use of polymerase chain reaction (PCR) and the comparison of the amplified alleles. We observed significative LOH (>20%) in the chromosomal regions of 2q14 (33.33%), 7q35 (24.00%), 10q22.1 (37.50%), 11q13–q14 (44.12%), 15q26 (40.63%), 17p13 (25.71%), and 17q21.3 (37.04%). We defined a 1-cM minimal common deletion in 11q13–q14 between D11S911 and D11S937 markers. A statistical analysis revealed a positive correlation between LOH of 11q13–q14 and clinicopathological data.
