Author/Authors :
Kucerova، نويسنده , , Lucia and Feketeova، نويسنده , , Lucia and Matuskova، نويسنده , , Miroslava and Kozovska، نويسنده , , Zuzana and Janega، نويسنده , , Pavol and Babal، نويسنده , , Pavel and Poturnajova، نويسنده , , Martina، نويسنده ,
Abstract :
The extent of local bystander effect induced by fusion yeast cytosine deaminase::uracil phosphoribosyltransferase (yCD) in combination with 5-fluorocytosine (5FC) was evaluated in xenogeneic model of human medullary thyroid carcinoma (MTC). This approach to gene-directed enzyme/prodrug therapy (GDEPT) induces strong bystander cytotoxicity. Effector yCD-TT mixed with target EGFP-TT cells in a ratio 2:9 could achieve significant tumor regression and 14-fold decrease in serum marker calcitonin upon 5FC administration. Histopathological analysis unraveled that antitumor effect resulted in tumor dormancy and proliferation arrest of remaining tumor cell clusters in vivo. yCD/5FC combination represents another GDEPT approach to achieve tumor growth control in MTC.
Keywords :
Human medullary thyroid carcinoma , Fusion yeast cytosine deaminase , Gene-directed enzyme/prodrug therapy , 5-fluorocytosine , Bystander effect
