Author/Authors :
Cao، نويسنده , , Qiag and Abeysinghe، نويسنده , , Harindra and Chow، نويسنده , , Ohn and Xu، نويسنده , , Jia and Kaung، نويسنده , , Hui-Lee and Fong، نويسنده , , Chin-To and Keng، نويسنده , , Peter and Insel، نويسنده , , Richard A and Lee، نويسنده , , Wyan-Ching Mimi and Barrett، نويسنده , , J.Carl and Wang، نويسنده , , Nancy، نويسنده ,
Abstract :
To determine the pathogenic role of chromosomes 11 and 17 in the carcinogenesis of human ovarian cancers, neoR-tagged chromosome 11 or 17 was transferred from cell lines A9H11 or A9H17, respectively, into the ovarian carcinoma cell line SKOV-3 using microcell-mediated chromosome transfer. The chromosome transfer was verified by polymerase chain reaction detection of the neoR gene, fluorescence in situ hybridization detection of an extra chromosome 11, and microsatellite polymorphism detection of an exogeneous chromosome 11. Five SKOV-3/A9H11 hybrids and five SKOV-3/A9H17 hybrid clones were generated. For the chromosome 11 transfer, complete suppression of tumorigenicity was observed in four clones, (11)9-8 and 11(H)7-2, 11(H)8-3, and 11(H)7-2, 100 days post implantation. For the chromosome 17 transfer, no complete suppression of tumorigenicity was observed. However, an increased latency period ranging from 25 to 49 days in contrast to 7 days for the SKOV-3 parental line, and a significant reduction in tumor size was observed. There was no correlation between the in vitro growth rate and the tumorigenicity or length of latency period. Our results demonstrate functionally that chromosome 11 may carry a tumor suppressor gene(s) while chromosome 17 may carry a tumor growth-inhibitor gene(s) for the ovarian carcinoma cell line, SKOV-3.
