Author/Authors :
Araْjo، نويسنده , , Thaise G. and Paiva، نويسنده , , Carlos E. and Rocha، نويسنده , , Rafael M. and Maia، نويسنده , , Yara C.P. and Sena، نويسنده , , Angela A.S. and Ueira-Vieira، نويسنده , , Carlos and Carneiro، نويسنده , , Ana Paula and Almeida، نويسنده , , Juliana F. and de Faria، نويسنده , , Paulo R. and Santos، نويسنده , , Donizeti W. and Calلbria، نويسنده , , Luanda and Alcântara، نويسنده , , Tânia M. and Soares، نويسنده , , Fernando A. and Goulart، نويسنده , , Luiz R.، نويسنده ,
Abstract :
The discovery of novel markers for breast cancer (BC) has been recently relied on antibody combinatorial libraries and selection through phage display. We constructed a recombinant Fab library, and after selections against BC tissues, the FabC4 clone was thoroughly investigated by immunohistochemistry in 232 patients with long-term follow-up. The FabC4 ligand was determined by mass spectrometry. The FabC4 expression was associated with younger age, lack of progesterone receptor, higher histological grades and non-luminal subtypes, and it also identified a subset of good prognostic triple-negative BCs, possibly targeting a conformational epitope of Cytokeratin-10 (CK10). This new CK10-epitope specific antibody may open new possibilities in diagnostic and therapeutic strategies.
Keywords :
combinatorial library , breast cancer , phage display , Recombinant Antibody
