Author/Authors :
Mart??nez-Ram??rez، نويسنده , , A and Rodr??guez-Perales، نويسنده , , S and Meléndez، نويسنده , , B and Mart??nez-Delgado، نويسنده , , B and Urioste، نويسنده , , M and Cigudosa، نويسنده , , J.C and Ben??tez، نويسنده , , J، نويسنده ,
Abstract :
The A673 cell line was established from a patient with a primary rhabdomyosarcoma (RMS), which is referred to in the literature either as a Ewing tumor (ET) or as RMS. Although the two tumoral types are associated with specific and well-characterized translocations, no cytogenetic report on this cell line has been published. We characterized the A673 cell line using a combination of spectral karyotyping (SKY), fluorescence in situ hybridization (FISH), and reverse transcriptase polymerase chain reaction (RT-PCR), which revealed the presence of a complex karyotype and a translocation involving chromosomes 11 and 22 and the fusion of EWS and FLI1 genes, both events being specific to ET. Neither cytogenetics nor molecular alterations specific to RMS were found.
