Detection of genetic alterations in Korean ovarian carcinomas by degenerate oligonucleotide primed polymerase chain reaction–comparative genomic hybridization

Chromosomal aberrations in 22 Koreans with ovarian carcinomas were investigated by degenerate oligonucleotide primed–polymerase chain reaction comparative genomic hybridization. The common sites of copy number increases were 20q (90%), 17q23∼qter (86%), 8q22∼qter (68%), 3q25∼qter (59%), 6p21 (59%), 11q13 (54%), 16p (40%), 2q31∼qter (36%), 7q (36%), 14q31 (36%), 15q24∼qter (36%), and 1q32∼qter (31%). DNA amplification was identified in 18 carcinomas (82%). The frequent sites of amplification were 20q13.2∼qter, 8q24.1, 17q23∼qter, 3q25∼qter, and 6p21. The most frequent sites of copy number decreases were 4q21∼q31 (54%), 5q13∼q21 (50%), and 13q14∼q21 (45%). The recurrent gains and losses of chromosomal regions identified in this study provide candidate regions that may contain oncogenes or tumor suppressor genes, respectively.