Author/Authors :
Bonne، نويسنده , , Anita and Vreede، نويسنده , , Lilian and Kuiper، نويسنده , , Roland P. and Bodmer، نويسنده , , Danielle and Jansen، نويسنده , , Corine and Eleveld، نويسنده , , Marc and van Erp، نويسنده , , Femke and Arkesteijn، نويسنده , , Ger and Hoogerbrugge، نويسنده , , Nicoline and van Ravenswaaij، نويسنده , , Conny and Schoenmakers، نويسنده , , Eric F.P.M. and Geurts van Kesse، نويسنده ,
Abstract :
Our group and others had previously developed a high throughput procedure to map translocation breakpoints using chromosome flow sorting in conjunction with microarray-based comparative genomic hybridization (arrayCGH). Here we applied both conventional positional cloning and integrated arrayCGH procedures to the mapping of constitutional chromosome anomalies in four patients with renal cell cancer (RCC), three with a chromosome 3 translocation, and one with an insertion involving chromosome 3. In one of these patients, who was carrying a t(3;4)(p13;p15), the KCNIP4 gene was found to be disrupted. KCNIP4 belongs to a family of potassium channel–interacting proteins and is highly expressed in normal kidney cells. In addition, KCNIP4 splice variants have specifically been encountered in RCC.
