In vivo diffusion characteristics of rat spinal cord

Complete apparent diffusion tensor (ADTs) of spinal cord was measured in vivo in nine rats at 2.0 T. Two rotationally invariant parameters, the trace, which is a measure of the mean diffusivity, and the lattice index (LI), which reflects the degree of orientation coherence of tissue, have been estimated from the ADT. The mean white matter (WM) trace value (3.05 ± 0.26 mm2/sec) was found to be substantially higher than the gray matter (GM) trace (2.36 ± 0.39 mm2/sec), in contrast with the published results on fixed, excised cord. Statistically significant anisotropic diffusion was observed in both WM and GM, with greater anisotropy in the WM (LI = 0.67 ± 0.06) than in the GM (LI = 0.51 ± 0.05).