A one step derivatization of controlled pore glass for oligonucleotide solid-phase synthesis

A one step anchoring of various diols on bare controlled pore glass (CPG) support via an adsorption mechanism allowed us to synthesize different (oligonucleotide–3′-diol) conjugates with high purity, through standard phosphoramidite chemistry. Diol loading on CPG proved to be efficient and reproducible. This methodology virtually allows the synthesis of any 3′-modified oligonucleotide, using any reporter molecule containing a diol moiety adsorbed on the CPG. Moreover, vicinal diol as glycol or glycerol enabled the release of some part of oligonucleotide-3′-OH.