Synthesis and ESR studies of nitronyl nitroxide-tethered oligodeoxynucleotides

We report on the development of a nucleoside labeled with a nitronyl nitroxide group, NNU, and the synthesis of oligodeoxynucleotides containing NNU. The spin signals of NNU-containing oligodeoxynucleotides varied with the degree of hybridization of the complementary strand and the distance between nitronyl nitroxide spins.