Author/Authors :
Shotorbani، نويسنده , , Siamak Sandoghchian and He، نويسنده , , Zhiqiang and Yang، نويسنده , , Heng and Sun، نويسنده , , Qiang and Xu، نويسنده , , Yan and Su، نويسنده , , Zhaoliang and Xue، نويسنده , , Yuan and Zheng، نويسنده , , Dong and Zhang، نويسنده , , Yun and Wang، نويسنده , , Shengjun and Shao، نويسنده , , Qixiang and Lu، نويسنده , , Liwei and Xu، نويسنده , , Huaxi، نويسنده ,
Abstract :
Hlx as a Th1-specific transcription factor, it appears to drive maturation of Th1 and IFN-γ secretion in cooperation with T-bet. In this study, we established a stable Hlx-over-expressed dendritic cell line (DC2.4/Hlx), and investigated the possible effect of Hlx gene on maturation of dendritic cell-line (DC2.4). Results shown that over-expressed Hlx in DC2.4 up-regulated the transcription and expression of IFN-γ, increased the expression of maturation makers including CD40, CD80, CD86, MHC-I and MHC-II. Functional assay for DC2.4/Hlx showed that over-expressed Hlx increased the expression level of interleukin-12 in the supernatant and decreased DC endocytosis when cells were incubated in vitro. Furthermore, using a syngeneic T cell activation model, we found that DC2.4/Hlx could obviously present ovalbumin (OVA) antigen to T cell in OVA pre-immunized mice.
Keywords :
Antigen presenting , dendritic cell , genetic modification , H2.0-like homebox
