Author/Authors :
Koizumi، نويسنده , , Tamio and Ochi، نويسنده , , Yutaka and Imoto، نويسنده , , Shion and Sakai، نويسنده , , Nobuo and Takao، نويسنده , , Shintaro and Kobayashi، نويسنده , , Seiichi and Matsuoka، نويسنده , , Masao and Sakano، نويسنده , , Hitoshi and Rüther، نويسنده , , Ulrich and Tokuhisa، نويسنده , , Takeshi، نويسنده ,
Abstract :
We examined effects of deregulated c-fos on the proliferation and differentiation of B cells in vitro, using splenic B cells from H2-c-fos transgenic mice. When these cells were cultured with lipopolysaccharide (LPS) plus recombinant interleukin 4 (rIL-4) (104 U/ml), the proliferative response of the B cells was augmented with any dose of LPS used. This augmented proliferation resulted in an increase in IgG1 production in the culture, at the lower concentrations of LPS (<2.5 μg/ml). However, H2-c-fos B cells did not produce IgG1 in the culture at higher concentrations of LPS (>5 μg/ml) probably due to the perturbation of B cell differentiation to antibody-forming cells. These results suggest that the deregulated expression of c-fos modulates the proliferation and differentiation B cells stimulated with LPS plus rIL-4.
