Title of article :
A Unique Murine CD43 Epitope Lp-3: Distinct Distribution from Another CD43 Epitope S7
Author/Authors :
Shiota ، نويسنده , , Yong-jun and Nishimura، نويسنده , , Hiroyuki and Okamoto، نويسنده , , Hiroshi and Yu، نويسنده , , Bo and Hattori، نويسنده , , Susumu and Abe، نويسنده , , Masaaki and Okada، نويسنده , , Takashi and Nozawa، نويسنده , , Shingo and Tsurui، نويسنده , , Hiromichi and Hirose، نويسنده , , Sachiko and Shirai، نويسنده , , Toshikazu، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1994
Pages :
12
From page :
402
To page :
413
Abstract :
In foregoing studies, we found a unique B cell differentiation antigen Lp-3 which is expressed on pre-B and premature B cells in the bone marrow, but is negative on bone marrow mature B cells and peripheral resting B cells. Nonetheless, Lp-3 was clearly positive on the majority of CD5 B(BI) cells. When we examined the biochemical nature and partial amino acid sequences of purified 132-kDa Lp-3 molecules and the nucleotide sequence of the cDNA clones, we found that Lp-3 is an epitope of CD43. Thus, the monoclonal antibody (mAb) Lp-3 may be the first mAb to murine CD43 defined by primary target structure analysis. Comparison of tissue distribution of Lp-3 and S7, an epitope previously suggested to associate with murine CD43, showed that they were similarly distributed on thymocytes, peripheral B and T cells, granulocytes, and platelets. In the bone marrow, while both Lp-3 and S7 were negative on mature B cells, the former was positive on all B lineage cells at an early ontogeny and the latter was positive only on the minor population of pre-B cells and pro-B cells. Lp-3 and S7 epitopes also showed different distributions on basement membranes of renal glomerulus, bronchus, and endometrium, lining cells of choroid plexus and muscular cells of arterioles in a variety of tissues. As CD43 has various isoforms generated by different degrees of glycosylation of the common core peptide, it is likely that Lp-3 and S7 are associated with different CD43 isoforms.
Journal title :
Cellular Immunology
Serial Year :
1994
Journal title :
Cellular Immunology
Record number :
1850168
Link To Document :
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