Title of article :
Detection of antibodies against SARS-CoV in serum from SARS-infected donors with ELISA and Western blot
Author/Authors :
Wang، نويسنده , , Yue-Dan and Li، نويسنده , , Yan and Xu، نويسنده , , Guo-Bin and Dong، نويسنده , , Xue-Yuan and Yang، نويسنده , , Xiao-Ang and Feng، نويسنده , , Zhen-Ru and Tian، نويسنده , , Chan and Chen، نويسنده , , Wei Feng، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2004
Pages :
6
From page :
145
To page :
150
Abstract :
Recombinant fragments of S proteins from the Severe Acute Respiratory Syndrome (SARS) coronavirus (SARA-CoV) were generated and used in a Western blot (WB) assay that was compared to a commercial SARS ELISA method. In 85% of confirmed SARS cases (n = 20), the S2 recombinant fragment based WB was positive and this was comparable to the commercial ELISA using heat killed SARS-CoV. WB using the other four recombinant fragments in confirmed SARS cases generated lower rates of detection (S1—75%, S1-N—25%, S1-C—55%). Evaluation of sera from healthy controls (n = 60) resulted in two weakly positive ELISA results with the remainder being negative while the S2 protein WB demonstrated three positive results from the 20 controls with a history of SARS contact and no positive results in 40 noncontact controls. A discrepancy between the ELISA and S2 WB arose when evaluating per-2003 sera from individuals (n = 10) with SARS-like symptoms (ELISA—100% positive, S2 WB—30% positive). These data suggest that the S2 WB assay may be particularly useful in ELISA-negative SARS cases and in some ELISA-positive non-SARS cases.
Keywords :
SARS , ELISA , Antibody , wb , immune response
Journal title :
Clinical Immunology
Serial Year :
2004
Journal title :
Clinical Immunology
Record number :
1850906
Link To Document :
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