Naive (CD45RA+) T Lymphocytes Are More Sensitive to Oxidative Stress-Induced Signals Than Memory (CD45RO+) Cells

Formation of reactive oxygen intermediates (ROI) after oxidative stress has been shown to be an activation signal for T lymphocytes, e.g., expression of IL-2 and its receptor are induced. These ROI-induced effects can, to a large extent, be attributed to the activation of the transcription factor NF-κB. Now we have examined whether naive and memory T lymphocytes differ in their sensitivity to ROI-mediated signals. When CD45RA+(naive) and CD45RO+(memory) T lymphocytes were directly stimulated with H2O2, NF-κB nuclear translocation was stronger in naive cells than in memory cells and it could be induced with lower doses. The composition of the induced nuclear NF-κB (levels of p50 and RelA proteins) was similar in these cell types. The magnitude and kinetics of intracellular ROI were similar, suggesting that there were no differences in ROI-forming mechanisms or antioxidative capacities. The probable regulatory point was the cytoplasmic IκB inhibitor: in CD45RA+cells, H2O2caused a more profound depression in the levels of IκBα. These findings indicate that T cells representing different activation and/or differentiation stages can be differ- entially responsive to ROI-mediated signals.