Title of article :
Dendritic cells pulsed with keyhole limpet hemocyanin and cryopreserved maintain anti-tumor activity in a murine melanoma model
Author/Authors :
Lin and Teitz-Tennenbaum، نويسنده , , Seagal and Li، نويسنده , , Qiao and Davis، نويسنده , , Mary A. and Chang، نويسنده , , Alfred E.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2008
Pages :
10
From page :
482
To page :
491
Abstract :
We compared viability, phenotype, in vitro function and therapeutic efficacy of murine unpulsed-dendritic cells (-DC), DC pulsed with keyhole limpet hemocyanin (KLH-DC) and cryopreserved KLH-DC (C-KLH-DC). Mean viability (% ± SE) of unpulsed-DC, KLH-DC and C-KLH-DC was 93.6 ± 0.9, 93.9 ± 0.8 and 87.4 ± 1.6, respectively. Pulsing DC with KLH did not induce maturation or affect in vitro function. Cryopreservation of KLH-DC reduced MHC I, CD80 and CD86 expression, endocytic capacity and allogeneic splenocyte stimulatory capacity. Intratumoral (i.t.) vaccination of mice bearing s.c. D5 melanoma with unpulsed-DC, KLH-DC or C-KLH-DC elicited comparable anti-tumor immune responses and inhibited tumor growth to the same extent. Combining radiotherapy with i.t. unpulsed-DC, KLH-DC or C-KLH-DC administration enhanced induction of anti-tumor immune responses and inhibition of tumor growth to a similar degree. Cryopreservation of KLH-DC slightly reduces viability, expression of co-stimulatory cell surface markers and in vitro function; however, in vivo anti-tumor activity is fully maintained with or without radiotherapy.
Keywords :
keyhole limpet hemocyanin , dendritic cells , Cellular immunotherapy , Cancer vaccines , Cryopreservation
Journal title :
Clinical Immunology
Serial Year :
2008
Journal title :
Clinical Immunology
Record number :
1853674
Link To Document :
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