Author/Authors :
Brehm، نويسنده , , Michael A. and Cuthbert، نويسنده , , Amy and Yang، نويسنده , , Chaoxing and Miller، نويسنده , , David M. and DiIorio، نويسنده , , Philip and Laning، نويسنده , , Joseph and Burzenski، نويسنده , , Lisa and Gott، نويسنده , , Bruce and Foreman، نويسنده , , Oded and Kavirayani، نويسنده , , Anoop and Herlihy، نويسنده , , Mary and Rossini، نويسنده , , Aldo A. and Shultz، نويسنده , , Leonard D. an، نويسنده ,
Abstract :
“Humanized” mouse models created by engraftment of immunodeficient mice with human hematolymphoid cells or tissues are an emerging technology with broad appeal across multiple biomedical disciplines. However, investigators wishing to utilize humanized mice with engrafted functional human immune systems are faced with a myriad of variables to consider. In this study, we analyze HSC engraftment methodologies using three immunodeficient mouse strains harboring the IL2rγnull mutation; NOD-scid IL2rγnull, NOD-Rag1null IL2rγnull, and BALB/c-Rag1null IL2rγnull mice. Strategies compared engraftment of human HSC derived from umbilical cord blood following intravenous injection into adult mice and intracardiac and intrahepatic injection into newborn mice. We observed that newborn recipients exhibited enhanced engraftment as compared to adult recipients. Irrespective of the protocol or age of recipient, both immunodeficient NOD strains support enhanced hematopoietic cell engraftment as compared to the BALB/c strain. Our data define key parameters for establishing humanized mouse models to study human immunity.
Keywords :
scid , Hematopoietic Stem Cells , IL-2R “common” gamma chain , Humanized mice
