Author/Authors :
Savilahti، نويسنده , , Emma M. and Karinen، نويسنده , , Sirkku and Salo، نويسنده , , Harri M. and Klemetti، نويسنده , , Paula and Saarinen، نويسنده , , Kristiina M. and Klemola، نويسنده , , Timo and Kuitunen، نويسنده , , Mikael and Hautaniemi، نويسنده , , Sampsa and Savilahti، نويسنده , , Erkki and Vaarala، نويسنده , , Outi، نويسنده ,
Abstract :
The role of T regulatory cells in spontaneous recovery from cowʹs milk allergy (CMA) is unclear. We investigated the mRNA expression of 12 T-cell markers and the protein expression of CD4, CD25, CD127, FoxP3 after in vitro β-lactoglobulin stimulation of peripheral blood mononuclear cells from children with persisting CMA (n = 16), early recovery (n = 20) or no atopy (n = 21). Artificial neural networks with exhaustive search for all marker combinations revealed that markers FoxP3, Nfat-C2, IL-16 and GATA-3 distinguished patients with persisting CMA most accurately from other study groups. FoxP3 mRNA expression following β-lactoglobulin stimulation was highest in children with persisting CMA. Also the FoxP3 intensity in CD4+ CD25highCD127low cells was higher in children with CMA compared with non-atopic children. The expression profile of both Th2- and T regulatory cell-related genes thus reflects the clinical activity of CMA. Tolerance, in contrast, is not characterized by activation of circulating T regulatory cells.
