Subtiligase as a hydrothiolase for the synthesis of peptide thioacids

Subtiligase catalyzes the hydrolysis or the aminolysis of a peptide glycolate ester substrate via an acyl-enzyme thioester intermediate. We show that this intermediate can be intercepted by a hydrosulfide ion to generate a peptide thioacid as the hydrothiolysis product. Also shown is the use of the so-prepared peptide thioacids in mini thiol capture ligation.