Author/Authors :
Dourado، نويسنده , , Luana Pereira Antunes and Saldanha، نويسنده , , Janaيna Clلudia da Silva and Gargiulo، نويسنده , , Daniela Longo and Noviello، نويسنده , , Maria de Lourdes Meirelles and Brant، نويسنده , , Clلudia Caldeira and Reis، نويسنده , , Maria Letيcia Costa and Souza، نويسنده , , Raphaela Mendes Fernandes de and Faria، نويسنده , , Ana Maria Caetano and Souza، نويسنده , , Danielle da Glَria de and Cara، نويسنده , , Denise Carmona Cara، نويسنده ,
Abstract :
To ascertain the role of IL-4 in aversion to antigen induced by food allergy, wild type and IL-4 deficient BALB/c mice were sensitized with ovalbumin and challenged orally with egg white. Sensitized wild type mice had increased production of IL-4 by spleen and mesenteric lymph node cells in vitro, higher levels of serum anti-ovalbumin IgE and IgG1, aversion to ingestion of the antigen and loss of body weight after continuous oral challenge. Intestinal changes in wild type sensitized mice included eosinophil infiltration and increased mucus production. The IL-4 deficiency impaired the development of food allergy and the aversion to antigen, suggesting the involvement of the antigen specific antibodies. When IL-4 deficient mice received serum from sensitized wild type donors, the aversion was restored. These results indicate that production of IL-4 and specific IgE/IgG1 antibodies correlate with aversion to antigen induced by food allergy in mice.
Keywords :
IL-4 , food allergy , Aversion , mice , Eosinophil , Mucus secretion , IGE , IgG1 , Serum transfer , Ovalbumin
