Author/Authors :
Xiao، نويسنده , , Gang and Miyazato، نويسنده , , Akiko and Abe، نويسنده , , Yuzuru and Zhang، نويسنده , , Tiantuo and Nakamura، نويسنده , , Kiwamu and Inden، نويسنده , , Ken and Tanaka، نويسنده , , Misuzu and Tanno، نويسنده , , Daiki and Miyasaka، نويسنده , , Tomomitsu and Ishii، نويسنده , , Keiko and Takeda، نويسنده , , Kiyoshi and Akira، نويسنده , , Shizuo and Saijo، نويسنده , , Shinobu and Iwakura، نويسنده , , Yoichiro and Adachi، نويسنده , , Yoshiyuki and Ohno، نويسنده , , Naohito and Yamamoto، نويسنده , , Natsuo and Kunishima، نويسنده , , Hiroyuki and Hirakata، نويسنده , , Yoichi and Kaku، نويسنده , , Mitsuo and Kawakami، نويسنده , , Kazuyoshi، نويسنده ,
Abstract :
The mechanism by which host cells recognize Cordyceps sinensis, a Chinese herbal medicine that is known to exhibit immunomodulating activity, remains poorly understood. In this study, we investigated whether the DNA of this fungus could activate mouse bone marrow-derived dendritic cells (BM-DCs). Upon stimulation with C. sinensis DNA, BM-DCs released IL-12p40 and TNF-α and expressed CD40. Cytokine production and CD40 expression were attenuated by chloroquin and bafilomycin A. Activation of BM-DCs by C. sinensis DNA was almost completely abrogated in TLR9KO mice. According to a luciferase reporter assay, C. sinensis DNA activated NF-κB in HEK293T cells transfected with the TLR9 gene. Finally, a confocal microscopic analysis showed that C. sinensis DNA was co-localized with CpG-ODN and partly with TLR9 and LAMP-1, a late endosomal marker, in BM-DCs. Our results demonstrated that C. sinensis DNA caused activation of BM-DCs in a TLR9-dependent manner.
Keywords :
dendritic cells , DNA , TLR9 , Cordyceps sinensis , CD40 , cytokines
