Author/Authors :
Henriques، نويسنده , , Ana and Inês، نويسنده , , Luيs and Couto، نويسنده , , Maura and Pedreiro، نويسنده , , Susana and Santos، نويسنده , , Catarina and Magalhمes، نويسنده , , Mariana and Santos، نويسنده , , Paulo and Velada، نويسنده , , Isabel and Almeida، نويسنده , , Anabela and Carvalheiro، نويسنده , , Tiago and Laranjeira، نويسنده , , Paula and Morgado، نويسنده , , José Mلrio and Pais، نويسنده , , Maria Luيsa and Silva، نويسنده , , José Antَnio Pereira da and Paiva، نويسنده , , Artur، نويسنده ,
Abstract :
To compare frequency and functional activity of peripheral blood (PB) Th(c)17, Th(c)1 and Treg cells and the amount of type 2 cytokines mRNA we recruited SLE patients in active (n = 15) and inactive disease (n = 19) and healthy age- and gender-matched controls (n = 15). The study of Th(c)17, Th(c)1 and Treg cells was done by flow cytometry and cytokine mRNA by real-time PCR. Compared to NC, SLE patients present an increased proportion of Th(c)17 cells, but with lower amounts of IL-17 per cell and also a decreased frequency of Treg, but with increased production of TGF-β and FoxP3 mRNA. Ιn active compared to inactive SLE, there is a marked decreased in frequency of Th(c)1 cells, an increased production of type 2 cytokines mRNA and a distinct functional profile of Th(c)17 cells. Our findings suggest a functional disequilibrium of T-cell subsets in SLE which may contribute to the inflammatory process and disease pathogenesis.
Keywords :
Th1 , Tc17 , Regulatory T cells , Tc1 , Autoimmune Disease , SLE , Th17
