Enantioselective scavenging using homogenate of Rhodotorula graminis: a facile preparation of d-amino acid derivatives in enantiopure form

An enantioselective scavenger (ES) comprised homogenate of Rhodotorula graminis containing multiple enzymes can enantioselectively remove l-enantiomer in a racemic mixture of amino acid derivatives (AADs), yielding d-enantiomer in high ee. Thirteen non-proteinogenic AADs were produced in enantiopure d form. The method appears to be an efficient cleaning and preparative strategy which can be applied to the production of d-AADs in high ee by enantioselectively scavenging the ‘l-contaminants’.