Author/Authors :
Ramos، نويسنده , , Aline de Souza and Ribeiro، نويسنده , , Joyce Benzaquem and Vazquez، نويسنده , , Leonardo and Fiaux، نويسنده , , Sorele Batista and Leite، نويسنده , , Selma Gomes Ferreira and Ramos، نويسنده , , Maria da Conceiçمo Klaus V. and Neto، نويسنده , , Francisco Radler de Aquino and Antunes، نويسنده , , O.A.C.، نويسنده ,
Abstract :
The enantioselective reduction of ethyl benzoylacetate (EBA) into ethyl (S)-3-hydroxy-3-phenylpropanoate (S-HPPE) by nine yeast strains and three filamentous fungi strains is described. The conversion obtained was in the range 0–89% and the enantiomeric excess was 100% in many cases. Conversion levels were higher when the reduction was performed with microorganisms immobilized in calcium alginate and enantioselectivity remained excellent. Some reaction’s conditions of bioreduction by immobilized cells of Rhodotorula rubra were studied using a 25-2 fractional factorial design.
Keywords :
?-Ketoester , Immobilization , Rhodotorula rubra , chiral reduction
