Single and double wavelength excitation of laser-induced fluorescence of normal and atherosclerotic peripheral vascular tissue

Laser-induced fluorescence spectra were recorded from the exposure of peripheral vascular tissue to both helium–cadmium and argon-ion laser radiation. Spectral analysis was based on simple algebraic expressions constructed using the intensity difference of the various spectral regions. The above methods were developed in order to determine the degree of atherosclerosis according to the laser-induced fluorescence signal. Similar results with single wavelength excitation were observed during in vivo irradiation of peripheral vessels.