Spectral analysis of laser-induced ultraweak delayed luminescence in cultured normal and tumor human cells: temperature dependence

The emission spectrum of ultraviolet A laser induced ultraweak delayed luminescence in cell cultures of mammalian cells depended on the temperature during irradiation and photonic measurements. A new method using a sophisticated photomultiplier system was developed in order to find differences between normal and tumor cells. The maximal peak of the emitted light for cultures measured at low temperature of 10 °C was near 510 nm in the green visible region while following irradiation at 32 °C this maximum was shifted to yellow-orange at 570 nm both in normal and melanoma cells. Overall, this ultraweak photonic model of cultured cells provides to be a new and powerful non-invasive tool for developing new strategies in skin cancer detection.