Title of article
Development of screening methods for transketolase activity and substrate scope
Author/Authors
Ranoux، نويسنده , , Adeline and Arends، نويسنده , , Isabel W.C.E. and Hanefeld، نويسنده , , Ulf، نويسنده ,
Issue Information
هفته نامه با شماره پیاپی سال 2012
Pages
4
From page
790
To page
793
Abstract
A new GC-based method is developed for the screening of activity and stereoselectivity of a wide range of polyols as substrates of transketolase (TK). Compared to previous screening methods, it shows higher reproducibility, sensitivity and range of detection. In combination with HPLC screening, it can be used efficiently to test mutant libraries obtained by directed evolution methods.
Keywords
Substrate scope , Mutant libraries , Transketolase , Screening methods , Selectivity
Journal title
Tetrahedron Letters
Serial Year
2012
Journal title
Tetrahedron Letters
Record number
1880094
Link To Document