Title of article :
Site-specific incorporation of 5-methylaminomethyl-2-thiouridine and 2-thiouridine(s) into RNA sequences
Author/Authors :
Leszczynska، نويسنده , , Grazyna and Pi?ta، نويسنده , , Jakub and Leonczak، نويسنده , , Piotr and Tomaszewska، نويسنده , , Agnieszka and Malkiewicz، نويسنده , , Andrzej، نويسنده ,
Issue Information :
هفته نامه با شماره پیاپی سال 2012
Abstract :
We present a reproducible protocol for the site-specific incorporation of 5-methylaminomethyl-2-thiouridine (mnm5s2U) into a model RNA fragment and, together with 2-methyladenosine (m2A), into the native sequence of the Escherichia coli tRNAGlu2 anticodon arm (E. coli ASLGlu2). This approach is also utilized for the synthesis of oligomers modified with multiple 2-thiouridines.
Keywords :
2-Thiouridine (s2U) , 5-Methylaminomethyl-2-thiouridine (mnm5s2U) , oligoribonucleotides , 2-Methyladenosine (m2A) , tRNAs modified components , solid-phase synthesis
Journal title :
Tetrahedron Letters
Journal title :
Tetrahedron Letters
