Author/Authors :
Chen، نويسنده , , Ke and Wang، نويسنده , , Wei-Min Qu، نويسنده , , Dezhong and Zhao، نويسنده , , Haoting and Xiong، نويسنده , , Wei and Luo، نويسنده , , Caijie and Yin، نويسنده , , Menghui and Zhang، نويسنده , , Biliang، نويسنده ,
Abstract :
A new 2′-OH protecting group, {[2,2-dimethyl-2-(2-nitrophenyl)acetyl]oxy} methyl (DAM), has been successfully applied to RNA oligonucleotides synthesis with high coupling efficiency. The 2′-O-DAM method is fully compatible with reagents and conditions employed in automated solid phase synthesis of RNA. Moreover, it can be easily removed via reduction in near-neutral solution.
Keywords :
Chemical modification , Solid phase synthesis of RNA , 2?-OH protecting group , siRNA , RNA oligonucleotides
