Phytochemical and Spectrophotometric Analyses of the Hill Toon, Cedrela Serrata Royle Methanolic Leaves Extract and Its Fractions

The present research was conducted to analyze the phytochemicals and spectrophotometry of the hill toon, Cedrela serrata Royle methanolic leaves extract and its fractions. Various phytochemicals such as alkaloids, carbohydrates (monosaccharides and disaccharides), flavonoids, steroids (phytosterols), saponins, tannins, phlobatannins, terpenoids, cardiac glycosides and anthraquinones were present in the methanolic extract (ME) as well as in fractions. Whereas monosaccharides and phlobatannins were weakly present in ME and aqueous fraction (AQF), while they were absent in case of n-butanol fraction (NBF) and ethyl acetate fraction (EAF). ME contained 5 compounds with first 2 compounds were present in high concentration while the remaining 3 were present in negligible concentration. They were absent in both AQF and EAF, whereas NBF showed the same compound with high concentration. In overall, the spectrophotometric analysis confirmed that good separation of the compounds had occurred among different fractions. The results concluded that the C. serrata extracts showed significant phytochemicals and spectrophotometric properties. It is suggested that further research should be needed on C. serrata to find out its active biological component.