Author/Authors :
Mirzaei، Tabassom نويسنده Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran Mirzaei, Tabassom , Alebouyeh، Masoud نويسنده , , Shokrzadeh، Leila نويسنده , , Asadzadeh Aghdaei، Hamid نويسنده Basic and Molecular epidemiology of Gastrointestinal Disorders Research Center, Shahid Beheshti University Medical Sciences, Tehran , , Farzi، Nasataran نويسنده Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran Farzi, Nasataran , zojaji، homayoun نويسنده shahid beheshti university , , Zali، Mohammad Reza نويسنده Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran Zali, Mohammad Reza
Arabic abstract :
Background: Helicobacter pylori diagnostic tests can be affected by different interventional factors. We studied the effects of smoking,
proton pump inhibitors (PPIs) and antibiotic administration on results of the bacterial culture, and its diagnosis tests due to polymerase
chain reaction (PCR) and rapid urease test (RUT) methods. Objectives: This study was aimed to investigate the undesirable effects of PPIs and antibiotics on the results of H. pylori screening tests in
patients with gastritis. Patients and Methods: A total of 100 patients with gastritis and indication for upper gastrointestinal (GI) endoscopy were enrolled
in this study. Three biopsy samples from each patient were immediately processed for detection of H. pylori based on culture, RUT, and
PCR methods. The sensitivity of these three detection methods was measured in the three infected patients groups that were subjected
to conventional therapy, proton pump inhibitors (PPIs) therapy, and no medication as control group. The possible effects of PPIs and
antibiotics on H. pylori detection were analyzed in vitro. Results: The prevalence of H. pylori infection was higher among the non-users and PPI users (40% and 57.9%, respectively), while the
number of isolated bacteria from the patients with a history of recent antibiotic prescription was significantly lower (18.75%) (P < 0.05). An
inverse association was found between H. pylori infection and smoking. Among the studied methods, PCR showed the highest sensitivity
in all groups. The results of RUT illustrated a significant difference between the PPI users and patients with a history of recent antibiotic
administration that was consistent with the results of in vitro study (P = 0.01). Conclusions: This study revealed a lower sensitivity of common H. pylori screening tests during the antibiotic or PPI administration. PCR
was determined as the most accurate test used for diagnosis of H. pylori infections
