Title of article
The VDR and TNF-α gene polymorphisms in Iranian tuberculosis patients: The study on host susceptibility
Author/Authors
Varahram، Mohammed نويسنده Mycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University, M.C., Tehran, Iran Varahram, Mohammed , Farnia، Parissa نويسنده , , Anoosheh، Sabar نويسنده Mycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University, M.C., Tehran, Ir Anoosheh, Sabar , Kazampour Dizaji، Mehdi نويسنده , , Merza، Muayad نويسنده Mycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University, M.C., Tehran, Iran Merza, Muayad , Saeif، Shima نويسنده Mycobacteriology Research Center, National Research Institute of Tuberculosis and Lung Diseases, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran Saeif, Shima , Masjedi، Mohammad-Reza نويسنده , , Velayati، Ali Akbar نويسنده Mycobacteriology Research Centre, National Research Institute of Tuberculosis and Lung Disease (NRITLD), Shahid Beheshti University, M.C., Tehran, Iran Velayati, Ali Akbar
Issue Information
فصلنامه با شماره پیاپی سال 2009
Pages
7
From page
207
To page
213
Abstract
Background: Vitamin–D receptor (VDR) and tumor necrosis factor–alpha (TNF-?) genes are thought to be important in
the intracellular killing of mycobacteria. This study aimed to determine the association of VDR and TNF-? variant with
development of pulmonary tuberculosis (PTB) among Iranian patients.
Patients and methods: Selected regions of VDR and TNF-? were amplified, and then the PCR products were digested
using restriction enzyme (RFLP). Digested products were run on 8% polyacrylamide gel, and were stained with silver–
nitrate. Single nucleotide polymorphisms (SNPs) at restriction sites of BsmI, and FokI of VDR gene and SNPs of TNF-?
at -238,-308, -244,-857,-863 positions were analyzed by PCR-RFLP among 117 PTB cases and 60 healthy controls.
Results: No statistically significant difference was observed in allele frequencies of FokI of VDR and TNF-? at -238,
-244,-863 and –857 position. Although, the frequency of b allele of BsmI (p=0.001) and -308 A variant in TNF-?
promoter region (p=0.006) were significantly more in PTB patients than healthy controls. The frequency of extended
diplotypes were different in patients and control subjects (p < 0.05).
Conclusion: This study confirmed the association of VDR BsmI and TNF-? -308A with susceptibility to tuberculosis in
Iranian PTB patients. In addition, the results showed the importance of haplotypes and diplotypes analysis in
determining the host susceptibility against TB
Journal title
Archives of Clinical Infectious Diseases
Serial Year
2009
Journal title
Archives of Clinical Infectious Diseases
Record number
1886182
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