Author/Authors :
Eslami، Gita نويسنده , , Taheri، Soudabeh نويسنده , , Baseri، Neda نويسنده Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran Baseri, Neda , Montazeri، Seyed Ali نويسنده Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran Montazeri, Seyed Ali , Shakeri، Abdolvahid نويسنده Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran Shakeri, Abdolvahid , Samadi، Roghayeh نويسنده Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran Samadi, Roghayeh , Dabiri، Hosein نويسنده Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran Dabiri, Hosein , Zahirnia، Zahra نويسنده Department of Microbiology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran , , Azargashb، Azanollah نويسنده 3Department of Community Medicine and Health, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran Azargashb, Azargashb
Abstract :
Background: Prevalence of Helicobacter pylori (H. pylori) differs among populations worldwide. H. pylori has been correlated with gastritis,
peptic ulcer and cancer. Considering the treatment failure due to antibiotic resistance, proper treatment of H. pylori infection is of great
importance. Objectives: The aim of this study was to determine the prevalence of H. pylori infection based on age, sex and also to examine antibiotic
resistance among patients referred to the endoscopy department of Shahid Beheshti University of Medical Sciences hospitals in Tehran
from 2010 to 2011. Patients and Methods: In this descriptive study, biopsies were taken from 192 patients with dyspepsia who underwent endoscopic
evaluation. For diagnosis of H. pylori infection and histologic assessment, samples were stained by Giemsa and hematoxylin-eosin (H &
E) staining. Based on the Sydney system for classification of gastritis, extent of H. pylori infection and severity of gastritis were measured.
To determine antibiotic resistance after culturing on Brucella agar medium, antibiogram test was done using modified disk diffusion
method on Mueller-Hinton agar considering standard conditions. Data analysis was done by the SPSS 16.0 software, using the chi-square
and T-test methods. Results: Overall prevalence of H. pylori was 83% among all patients and 90.3% among those with gastritis (CI 95% = 85.9% - 94.6%). There was
no significant correlation between the extent of H. pylori infection and age (P = 0.182) and gender (P = 0.466). Yet, there was a significant
correlation between H. pylori infection and severity of gastritis (P = 0.001). Rates of resistance to metronidazole, clarithromycin, amoxicillin,
ciprofloxacin and tetracycline were 50%, 16.1%, 5.2%, 4.6% and 3.6%, respectively. Conclusions: Prevalence of H. pylori infection in our patients was similar to that of developing countries. Severity of gastritis is correlated
with the extent of H. pylori infection. Moreover, the antibiotic resistance rate observed in this study signifies the ever-growing importance
of further antibiotic sensitivity studies to help with proper treatment regimens against H. pylori
