Author/Authors :
Rizzo، نويسنده , , Paula V.S. and Boarin، نويسنده , , Ligia A. and Freitas، نويسنده , , Ingridhy O.M. and Gomes، نويسنده , , Roberto S. and Beatriz، نويسنده , , Adilson and Rinaldi، نويسنده , , Andrelson W. and Domingues، نويسنده , , Nelson Luيs C.، نويسنده ,
Abstract :
This Letter introduces a new, cheap and green protocol for the thio-Michael reaction. Here we applied three free enzymes such as lipase from pancreas porcine, chymosin and papain and an immobilized one: the Liposyme®. The reactions were executed at room temperature and resulted in the thio-Michael adduct in good or excellent yields. The protocol describes the use of EtOH as solvent and a less percentage of enzymes, which is in concordance with the green chemistry topics, so we can mention that chymosin and papain were used as biocatalyst in an organic reaction for the first time in this Letter.
Keywords :
Biocatalysis , Free enzymes , Immobilized enzyme , Multi-gram scale , Thio-Michael
