Author/Authors :
Luo، نويسنده , , Shangwen and Kang، نويسنده , , Hahk-Soo and Krunic، نويسنده , , Aleksej and Chlipala، نويسنده , , George E. and Cai، نويسنده , , Geping and Chen، نويسنده , , Wei-Lun and Franzblau، نويسنده , , Scott G. and Swanson، نويسنده , , Steven M. and Orjala، نويسنده , , Jimmy، نويسنده ,
Abstract :
Two new (1 and 2) and three known (3–5) carbamidocyclophanes were isolated from a cultured freshwater cyanobacterium Nostoc sp. (UIC 10274) obtained from a sample collected at Des Plaines, Illinois. Their planar structures and stereoconfigurations were determined by extensive spectroscopic analysis including 1D/2D NMR experiments, HRESIMS as well as CD spectroscopy. Carbamidocyclophane F (1) showed potent anti-Mycobacterium tuberculosis activity in the microplate Alamar blue assay and low-oxygen-recovery assay with MIC values of 0.8 and 5.4 μM, respectively. Carbamidocyclophane F (1) also displayed antimicrobial activities against the gram positive bacteria Staphylococcus aureus and Enterococcus faecalis with MIC values of 0.1 and 0.2 μM, respectively. Carbamidocyclophane F (1) and Carbamidocyclophane G (2) both showed antiproliferative activity against MDA-MB-435 and HT-29 human cancer cell lines with IC50 values in the range from 0.5 to 0.7 μM.
