Effects of removal of seminal plasma on the survival rate of buffalo bull spermatozoa

To study the effect of removal of seminal plasma on survivability of spermatozoa at 37°C, three extenders, i.e. lactose-fructose-egg yolk-glycerol (A), sodium citrate-egg yolk (B) and milk-egg yolk-glycerol (C) were used. Analysis of variance for survivability revealed that there was a significant (P < 0.001) difference between treated (seminal plasma removed) and untreated (with seminal plasma) semen samples. Multiple mean comparisons of survivability and absolute index of survivability of extender C were significantly (P < 0.05) different from those of extenders A and B. The maximum survivability and absolute index of survivability were obtained with milk-egg yolk-glycerol extender.