Title of article :
Biological activity of the seminal plasma of alpacas: stimulus for the production of LH by pituitary cells
Author/Authors :
Paolicchi، نويسنده , , F. and Urquieta، نويسنده , , B. and Del Valle، نويسنده , , L. and Bustos-Obregَn، نويسنده , , E.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1999
Pages :
8
From page :
203
To page :
210
Abstract :
South American camelids are induced ovulators and require a stimulus to trigger the LH surge responsible for the ovulation. Seminal plasma (SP) of fertile alpacas (Lama pacos) was tested using a bioassay of pituitary cells to study the effect of seminal plasma on LH release. Plates containing rat pituitary cells (2×105 cells/90–95% viability) were cultured adding: (A) whole SP (WSP) treated with charcoal-dextran, or 1:2 or 1:4 proportions diluted in culture medium (DMEM/HEPES+antibiotics), or (B) 1:2 SP+anti-GnRH rabbit serum (inhibitory potency 10−5 M), or (C) 1:2 SP+anti-GnRH+100 nM synthetic GnRH (buserelin acetate) or (D) 100 nM, 50 nM, 10 nM, and 1 nM synthetic GnRH. Concentration (ng/ml) of LH secreted (Sec) and contained (Con) was analyzed using RIA 125I and the percentage of Sec and Con in each experiment was determined. The results of LH Sec for the cells treated with 50, 10, and 1 nM GnRH were 39, 13, and 1.5%, respectively (r2=98.41%, r=0.9920) but cells treated with 100 nM GnRH secreted 10% of LH. With WSP, 1:2, or 1:4 SP the LH Sec was of 44.5% (3.25 ng/ml), 27% (1.9 ng/ml), and 18% (1.2 ng/ml), respectively. The exposure of cells to 1:2 SP+anti-GnRH, or to 1:2 SP+anti-GnRH/100 nM GnRH produced 31% (2.20 ng/ml) and 30% (1.8 ng/ml) of LH Sec, respectively. These results suggest that the SP of alpacas could have some factor(s) different from GnRH that would contribute to the mechanisms of LH secretion and to the induced ovulation in the female alpaca.
Keywords :
In vitro culture adenohypophysis , LH , Alpaca , Seminal Plasma
Journal title :
Animal Reproduction Science
Serial Year :
1999
Journal title :
Animal Reproduction Science
Record number :
1905191
Link To Document :
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