Description of semen characteristics from six-banded armadillos (Euphractus sexcinctus) collected by electroejaculation

The aim of this study is to describe the characteristics of the semen from six-banded armadillos (Euphractus sexcinctus) collected by electroejaculation. Six mature males were physically restrained and electroejaculated twice for the collection of semen. Semen collected was immediately evaluated for appearance, volume, pH, sperm motility, vigor, morphology, percentage of live sperm and functional membrane integrity by light microscopy. Semen was obtained from all (100%) twelve attempts conducted for electroejaculation. Armadillos’ semen had a white-translucent appearance, and great viscosity. Mean values obtained in analysis of the semen were: 353 ± 86 μl for volume, 9 for pH, 45 ± 14 × 106 sperm/ml for concentration, 61 ± 7% motile sperm with 2 ± 0.2 for vigor, 55 ± 7% live sperm, 86 ± 2% morphologic normal sperm, and 46 ± 6% functional membrane integrity. In conclusion, semen from six-banded armadillos can be efficiently obtained by electroejaculation. The characteristics of semen collected by electroejaculation in six-banded armadillos provide background information that may be useful for assisted breeding programs in the members of the Xenarthra family.