Isolation and characterization of two soil derived yeasts for bioethanol production on Cassava starch

Two ethanol-producing yeast strains, CHY1011 and CHFY0901 were isolated from soil in South Korea using an enrichment technique in a yeast peptone dextrose medium supplemented with 5% (w v−1) ethanol at 30 °C. The phenotypic and physiological characteristics, as well as molecular phylogenetic analysis based on the D1/D2 domains of the large subunit (26S) rRNA gene and the internally transcribed spacer (ITS) 1 + 2 regions suggested that they were novel strains of Saccharomyces cerevisiae. During shaking flask cultivation, the highest ethanol productivity and theoretical yield of S. cerevisiae CHY1011 in YPD media containing 9.5% total sugars was 1.06 ± 0.02 g l−1 h−1 and 95.5 ± 1.2%, respectively, while those for S. cerevisiae CHFY0901 were 0.97 ± 0.03 g l−1 h−1 and 91.81 ± 2.2%, respectively. Simultaneous saccharification and fermentation for ethanol production was carried out using liquefied cassava (Manihot esculenta) starch in a 5 l lab-scale jar fermenter at 32 °C for 66 h with an agitation speed of 2 Hz. Under these conditions, S. cerevisiae CHY1011 and CHFY0901 yielded a final ethanol concentration of 89.1 ± 0.87 g l−1 and 83.8 ± 1.11 g l−1, a maximum ethanol productivity of 2.10 ± 0.02 g l−1 h−1 and 1.88 ± 0.01 g l−1 h−1, and a theoretical yield of 93.5 ± 1.4% and 91.3 ± 1.1%, respectively. These results suggest that S. cerevisiae CHY1011 and CHFY0901 have potential use in industrial bioethanol fermentation processes.